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Akt (磷酸化-Ser473)多克隆抗体

货号 品牌 CAS 规格 销售价(RMB) 折扣价(RMB) 备注 数量
ABP0030-100μL Abbkine 100μL 1998 1998
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商品信息

产品英文名称Akt (phospho Ser473) Polyclonal Antibody
免疫原合成多肽:人源Akt 磷酸化位点为中心:S473
宿主
反应性人, 大鼠, 小鼠
应用ELISA, IF, IHC-P, WB
实验建议最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为:WB:1:500-1:2000,IF:1:50-1:200,IHC-P:1:100-1:300,ELISA:1:40000。
克隆性多克隆
纯化工艺使用表位特异性的免疫原,通过亲和层析,从兔抗血清中亲和纯化抗体。

商品属性

产品形式液体溶液
浓度1 mg/ml
分子量55 KD
储存缓冲液PBS缓冲液(pH 7.4),含有0.5%BSA(稳定剂),0.02%叠氮化钠(防腐剂)和50%甘油。
保存建议自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
运输条件蓝冰运输
警告本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

附加信息

背景The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in AKT1 have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for AKT1.
基因ID207/208/10000
别名AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT2; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B
其它该抗体可检测内源蛋白
蛋白质IDP31749/P31751/Q9Y243